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is the prettiest.Chemical modification of natural alpha-bungarotoxin by lactoperoxidase and beta-naphthoflavone. alpha-Bungarotoxin was shown to be a specific inhibitor of acetylcholine release from the chick biventer cervicis nerve-muscle preparation. In order to determine the functional relevance of the N-terminal alpha-bungarotoxin domain and to identify amino acid residues which may be involved in the inhibition of acetylcholine release, the toxin was chemically modified by lactoperoxidase oxidation and beta-naphthoflavone. alpha-Bungarotoxin was fully inactivated by lactoperoxidase treatment (concentration dependence, optimum pH and time course). After 4 hours at pH 6.5 and 0 degrees C, 51% of the toxin activity was lost. An absorbance spectrum analysis of the oxidized toxin showed a decrease in the molar absorptivity of the chromophore of the toxin. beta-Naphthoflavone oxidation of alpha-bungarotoxin increased the toxin's nonenzymatic proteolytic susceptibility in the pH range 6-8 and decreased the molar absorptivity of the chromophore. Beta-Naphthoflavone treatment of toxin followed by proteolysis resulted in two distinct polypeptide species. One species consisted of the modified toxin and the proteolytic fragments was inactivated by alpha-chymotrypsin, but not by trypsin. The second species consisted of the proteolytic fragments and the modified toxin was fully inactivated by alpha-chymotrypsin. The results suggest that the alpha-bungarotoxin chromophore may be tyrosine-95, and that the tetrapeptide sequence -Glu-Tyr-Glu-Ala-NH2 may be essential for the activity of alpha-bungarotoxin.Effects of adjuvant therapy on cell-mediated immunity to autologous melanoma in patients with stage III melanoma. The aim of the present study was to analyze the effects of adjuvant therapy on the immune system of patients with advanced melanoma. We studied the effects of a recombinant interleukin 2 (rIL-2) therapy on the in vitro cellular immune response against autologous melanoma in 20 patients with stage III melanoma (UICC stage



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